目的 研究草苁蓉不同溶剂萃取物对对乙酰氨基酚(APAP)诱导的小鼠急性肝损伤的保护作用。方法 将实验小鼠随机分为正常组、模型组、联苯双脂组(阳性对照组)及草苁蓉不同溶剂萃取物组。每日给药1次, 连续7 d。实验末期, 腹腔注射对乙酰氨基酚建立急性肝损伤模型, 比色法检测血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、白蛋白(ALB)肝组织活性氧(ROS)、脂质过氧化物(LPO)、丙二醛(MDA)、还原型谷胱甘肽(GSH)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)、Na+-K+-ATP酶(Na+-K+-ATPase)、Ca2+-Mg2+-ATP酶(Ca2+-Mg2+-ATPase)、一氧化氮(NO)和诱导型一氧化氮合酶(iNOS)水平。结果 草苁蓉正丁醇萃取物和水萃取物显著降低对乙酰氨基酚诱发的急性肝损伤小鼠血清丙氨酸氨基转移酶、天冬氨酸氨基转移酶活性, 增高血清白蛋白水平, 减少肝组织活性氧、脂质过氧化物和丙二醛水平, 升高肝组织过氧化氢酶、谷胱甘肽过氧化物酶、超氧化物歧化酶活性谷胱甘肽水平, 升高肝线粒体Na+-K+-ATP酶和Ca2+-Mg2+-ATP酶活性(P＜0.05), 降低肝诱导型一氧化氮合酶活性和一氧化氮水平。结论 草苁蓉对对乙酰氨基酚诱导的小鼠急性肝损伤具有保护作用, 其机制可能与抗氧化和抗炎作用有关。
Abstract
OBJECTIVE To investigate the protective effect of different solvent fractions of Boschniakia rossica on acute liver injury induced by acetaminophen (APAP) in mice. METHODS The mice were randomly assigned to the normal control, model control, bifendate (positive control), as well as groups of different solvent fractions of Boschniakia rossica. Animals were treated once daily for 7 d. APAP were given intraperitoneally to the mice of groups, then the alanine aminotransferase (ALT), aspartate aminotransferase (AST), albumin (ALB), reactive oxygen species (ROS), lipid hydroperoxide (LPO), malondialdehyde (MDA), glutathione (GSH), catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), Na+-K+-ATPase, Ca2+-Mg2+-ATPase nitric oxide (NO) and inducible nitric oxide synthase (iNOS) were detected by the colorimetric method. RESULTS The administration with butanol soluble fraction and aqueous fraction of Boschniakia rossica reduced the serum ALT and AST activities, increased the serum ALB level, decreased the hepatic ROS, LPO and MDA levels, increased the CAT, GPx, SOD activities and GSH level, increased the Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities of liver mitochondria, and decreased hepatic iNOS activity and NO level of liver in mice with acute liver injury.CONCLUSION The different solvent fractions of Boschniakia rossica have protective effects on acute liver injury induced by APAP in mice, probably via anti-oxidative and anti-inflammatory activities.
关键词
草苁蓉 /
肝损伤 /
对乙酰氨基酚 /
抗氧化 /
抗炎
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Key words
Boschniakia rossica /
liver injury /
acetaminophen /
anti-oxidative /
anti-inflammatory
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中图分类号：
R285.5
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参考文献
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脚注
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基金
国家自然科学基金资助项目(30360113;81160539)
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